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高维流式细胞术用于解剖种植体组织的免疫功能分析
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Bioengenharia
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JoVE Journal Bioengenharia
High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
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高维流式细胞术用于解剖种植体组织的免疫功能分析

DOI:
10.3791/61767-v

•

08:21 min

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September 15, 2021

•

,
1Section on Immuno-Engineering, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health

Capítulos

  • 00:05Introduction
  • 00:40Cell Isolation
  • 02:50Cell Surface Marker Staining
  • 04:27Cytometer and Unmixing Setup
  • 05:37Results: Representative 14-Color FACS Staining on Mouse Tissue
  • 07:25Conclusion

Summary

Tadução automática

Tadução automática

从解剖的植入物中分离细胞并通过流式细胞术对其进行表征，可以显著有助于了解针对植入物的免疫反应模式。本文描述了一种从解剖植入物中分离细胞及其染色以进行流式细胞术分析的精确方法。

Tags

High-dimensional Flow CytometryImmune Function AnalysisBiomaterialsHost Immune ResponseImmune Cell InfiltrationEnzymatic DigestionCell IsolationCell CountingViability StainingFlow Cytometry

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