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JoVE Journal Cancer Research
Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
 

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Article DOI: 10.3791/61775-v • 07:03 min • October 2nd, 2020
October 2nd, 2020 •
1,2, 1,2, 1, 1,2, 1,2,3,4
1London Regional Cancer Program, 2Department of Anatomy & Cell Biology, Western University, 3Department of Oncology, Western University, 4Lawson Health Research Institute

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Resumo
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This experimental protocol describes the isolation of BCSCs from breast cancer cell and tissue samples as well as the in vitro and in vivo assays that can be used to assess BCSC phenotype and function.

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Isolation Functional Assessment Breast Cancer Stem Cells Cell And Tissue Samples In Vitro In Vivo Characterization Assays Molecular Events Tumorigenicity Metastasis Therapeutic Strategies Single Cell Suspension Cell Dissociation Solution Culture Medium ALDH Substrate Buffer Tissue Sample Surgical Blades Dissociation Buffer
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