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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

DOI:
10.3791/61823-v

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05:56 min

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November 12, 2020

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, , , , , , ,
1CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, 2Faculty of Biotechnology,University of Wroclaw, 3Faculty of Biological Sciences,University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Capítulos

  • 00:05Introduction
  • 00:36Daily Checkpoint
  • 01:31Spot Size Calibration
  • 02:36svFCS Data Acquisition
  • 03:56Results: Representative svFCS Diffusion Law and Total Cholesterol Comparison
  • 05:07Conclusion

Summary

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This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

Tags

Spot Variation Fluorescence Correlation SpectroscopyMolecular DiffusionPlasma MembraneLiving CellsConfocal MicroscopyLaser PowerRhodamine 6GAuto Correlation FunctionDiffusion TimeSvFCS AnalysisXY ScanZ Scan

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