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纳索尼亚维特里彭尼斯的 Rnai 和克里斯普基因编辑的普帕尔和成人注射
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Biologia
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JoVE Journal Biologia
Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis
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纳索尼亚维特里彭尼斯的 Rnai 和克里斯普基因编辑的普帕尔和成人注射

DOI:
10.3791/61892-v

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08:41 min

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December 04, 2020

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, , ,
1Division of Biological Sciences, Section of Cell and Developmental Biology,University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences,The Pennsylvania State University

Capítulos

  • 00:04Introduction
  • 01:04Nasonia Rearing
  • 02:19Alignment of White and Black Pupae
  • 03:21Needle Preparation
  • 03:53Adult Injection with Aspirator Tube
  • 05:04Adult Injection with Aspirator Tube
  • 06:32Post-Injection Care and Mutant Screening
  • 07:27Results: Survival and Efficiency Rate of RNAi and CRISPR/Cas9 via Pupal and Adult Injections
  • 07:52Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们将 纳索尼亚维特里彭尼斯 高效注射 pupal 和成人注射的方法描述为胚胎微注射的可访问替代品，通过 RNA 干扰 （RNAi） 或通过 CRISPR/Cas9 基因组编辑进行基因敲除，实现感兴趣的基因的功能分析。

Tags

Nasonia VitripennisParasitoid WaspPupal InjectionAdult InjectionRNAiCRISPRGene EditingFunctional GeneticsInsect ModelNon-canonical Model OrganismEmbryonic MicroinjectionGenetic Transformation

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