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Pupal- og voksenindsprøjtninger til RNAi- og CRISPR-genredigering i Nasonia vitripennis
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Biologia
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JoVE Journal Biologia
Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Pupal- og voksenindsprøjtninger til RNAi- og CRISPR-genredigering i Nasonia vitripennis

DOI:
10.3791/61892-v

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08:41 min

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December 04, 2020

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, , ,
1Division of Biological Sciences, Section of Cell and Developmental Biology,University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences,The Pennsylvania State University

Capítulos

  • 00:04Introduction
  • 01:04Nasonia Rearing
  • 02:19Alignment of White and Black Pupae
  • 03:21Needle Preparation
  • 03:53Adult Injection with Aspirator Tube
  • 05:04Adult Injection with Aspirator Tube
  • 06:32Post-Injection Care and Mutant Screening
  • 07:27Results: Survival and Efficiency Rate of RNAi and CRISPR/Cas9 via Pupal and Adult Injections
  • 07:52Conclusion

Summary

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Tadução automática

Her beskriver vi metoder til effektive pupal- og voksenindsprøjtninger i Nasonia vitripennis som tilgængelige alternativer til embryomikroinjektion, der muliggør funktionel analyse af gener af interesse ved hjælp af enten RNA-lyddæmpning via RNA-interferens (RNAi) eller gen knockout via CRISPR / Cas9 genomredigering.

Tags

Nasonia VitripennisParasitoid WaspPupal InjectionAdult InjectionRNAiCRISPRGene EditingFunctional GeneticsInsect ModelNon-canonical Model OrganismEmbryonic MicroinjectionGenetic Transformation

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