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1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry,University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 5California Institute for Quantitative Biosciences,University of California San Francisco, 6Chan Zuckerberg Biohub,University of California San Francisco, 7Department of Chemical & Biomolecular Engineering,University of California Berkeley, 8Helen Wills Neuroscience Institute,University of California Berkeley, 9Department of Mechanical Engineering,University of California Berkeley, 10Center for Cellular Construction,University of California San Francisco
Capítulos
- 00:05Introduction
- 00:30Photopattern DNA onto Aldehyde-Functionalized Slides
- 02:46Lift and Label Cells with Cholesterol-Modified DNA
- 04:21Pattern the DNA-labeled Cells and Transferring into Hydrogel for 3D Culture
- 07:22Results: Adhesion of CMO-Labeled Cells to DNA Patterns
- 08:32Conclusion
Aquí presentamos un protocolo para micropatrones de células a resolución unicelular utilizando adhesión programada por ADN. Este protocolo utiliza una plataforma de fotolitografía de sobremesa para crear patrones de oligonucleótidos de ADN en un portaobjetos de vidrio y luego etiqueta las membranas celulares con oligonucleótidos complementarios disponibles comercialmente. La hibridación de los oligos da como resultado la adhesión celularprogramada.
