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1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry,University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 5California Institute for Quantitative Biosciences,University of California San Francisco, 6Chan Zuckerberg Biohub,University of California San Francisco, 7Department of Chemical & Biomolecular Engineering,University of California Berkeley, 8Helen Wills Neuroscience Institute,University of California Berkeley, 9Department of Mechanical Engineering,University of California Berkeley, 10Center for Cellular Construction,University of California San Francisco
Capítulos
- 00:05Introduction
- 00:30Photopattern DNA onto Aldehyde-Functionalized Slides
- 02:46Lift and Label Cells with Cholesterol-Modified DNA
- 04:21Pattern the DNA-labeled Cells and Transferring into Hydrogel for 3D Culture
- 07:22Results: Adhesion of CMO-Labeled Cells to DNA Patterns
- 08:32Conclusion
Här presenterar vi ett protokoll till mikromönsterceller vid encellsupplösning med hjälp av DNA-programmerad vidhäftning. Detta protokoll använder en bänkfotografiplattform för att skapa mönster av DNA-oligonukleotider på en glasrutschbana och märker sedan cellmembran med kommersiellt tillgängliga kompletterande oligonukleotider. Hybridisering av oligos resulterar i programmerad cell vidhäftning.
