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1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences,Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences,University of Hertfordshire
Capítulos
- 00:04Introduction
- 00:46Slide Preparation and Cell Culture
- 02:08DNA Halo Preparation
- 03:22Two‐Dimensional Fluorescence In Situ Hybridization
- 06:47Results: DNA Halos for High‐Resolution Analysis of Genomic Interactions with the Nucleoskeleton
- 08:41Conclusion
A combinação de preparações de halo de DNA com hibridização in situ fluorescente permite a análise de alta resolução das interações genômicas com o nucleoesqueleto. O genoma anexado leva a sinais fluorescentes hibridizados localizados dentro dos núcleos residuais extraídos, enquanto o genoma não ligado está no halo de DNA ao redor dos núcleos residuais.
