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使用免疫荧光检测斑马鱼胚胎心脏PM_2.5诱发DNA损伤

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Using Immunofluorescence to Detect PM_2.5-induced DNA Damage in Zebrafish Embryo Hearts

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使用免疫荧光检测斑马鱼胚胎心脏PM_2.5诱发DNA损伤

DOI:

10.3791/62021-v

•

05:58 min

•

February 15, 2021

•

Yujie Huang*¹, Yizhou Tao*¹, Chang Cai, Jin Chen, Cheng Ji, Stanley Aniagu, Yan Jiang, Tao Chen

¹School of Public Health,Soochow University, ²Toxicology, Risk Assessment and Research Division,Texas Commission on Environmental Quality

Capítulos

00:06Introduction
00:34Morphological Observation of Zebrafish Embryos and Cardiac Dissection
01:40Immunofluorescence Assay
04:29Results: Cardiac Defects and DNA Damage of Zebrafish Embryos at 72 hpf
05:36Conclusion

Summary

Tadução automática

该协议使用免疫荧光检测检测斑马鱼胚胎解剖心脏中_PM2.5诱发的DNA损伤。

Tags

Keywords: Immunofluorescence PM2.5 DNA Damage Zebrafish Embryo Heart 8-hydroxydeoxyguanosine Gamma-H2AX Antibody Fixation Immunostaining Microscopy

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