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一种从人类多能干细胞中生成肾类器官的简化方法

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

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一种从人类多能干细胞中生成肾类器官的简化方法

DOI:

10.3791/62452-v

•

07:39 min

•

April 13, 2021

•

Aneta Przepiorski, Amanda E. Crunk, Teresa M. Holm, Veronika Sander, Alan J. Davidson, Neil A. Hukriede³

¹Department of Developmental Biology,University of Pittsburgh, School of Medicine, ²Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland, ³Center for Critical Care Nephrology,University of Pittsburgh, School of Medicine

Capítulos

00:04Introduction
00:59Setting up the Kidney Organoid Assay and Feeding by Half-Medium Change
02:49Transfer of Embryoid Bodies to Stage II Medium
04:49Transfer to Spinner Flask and Feeding
05:37Setting up a 6-Well Magnetic Stir Plate
06:14Results: Immunofluorescence of the Kidney Organoids
06:51Conclusion

Summary

Tadução automática

在这里，我们描述了一种从人类多能干细胞（hPSCs）生成肾类器官的方案。该方案在两周内产生肾脏类器官。所得的类肾类器官可以在大型旋转瓶或多孔磁力搅拌板中培养，以进行平行的药物测试方法。

Tags

Kidney Organoids Human Pluripotent Stem Cells HPSCs Cell Culture Renal Development Renal Disease Simplified Method Cell Differentiation Embryoid Bodies Cell Culture Techniques

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