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使用Trowell型器官培养研究牙科干细胞的调节
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells
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使用Trowell型器官培养研究牙科干细胞的调节

DOI:
10.3791/62462-v

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06:22 min

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July 08, 2021

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,
1Research Program in Developmental Biology, Institute of Biotechnology,University of Helsinki, 2Department of Oral and Maxillofacial diseases, Faculty of Medicine,University of Helsinki

Capítulos

  • 00:04Introduction
  • 00:50Incisor Dissection and Isolation of the Proximal End Tissue
  • 03:45Tissue Fixation
  • 04:19Results: Analysis of the Isolated Cervical Loops
  • 05:51Conclusion

Summary

Tadução automática

Tadução automática

Trowell型器官培养方法已被用于解开控制牙齿发育的复杂信号网络，最近还用于研究与持续生长的小鼠门牙干细胞有关的调节。荧光报告动物模型和实时成像方法有助于对牙科干细胞及其特定的生态位微环境进行深入分析。

Tags

Trowell-type Organ CultureDental Stem CellsStem Cell NicheIncisor ToothMandibleCervical LoopIn Vitro CultureStem Cell Regulation

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