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Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells

DOI:
10.3791/62462-v

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06:22 min

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July 08, 2021

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1Research Program in Developmental Biology, Institute of Biotechnology,University of Helsinki, 2Department of Oral and Maxillofacial diseases, Faculty of Medicine,University of Helsinki

Chapters

  • 00:04Introduction
  • 00:50Incisor Dissection and Isolation of the Proximal End Tissue
  • 03:45Tissue Fixation
  • 04:19Results: Analysis of the Isolated Cervical Loops
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

The Trowell-type organ culture method has been used to unravel complex signaling networks that govern tooth development and, more recently, for studying regulation involved in stem cells of the continuously growing mouse incisor. Fluorescent-reporter animal models and live-imaging methods facilitate in-depth analyses of dental stem cells and their specific niche microenvironment.

Tags

Trowell-type Organ CultureDental Stem CellsStem Cell NicheIncisor ToothMandibleCervical LoopIn Vitro CultureStem Cell Regulation

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