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Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
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Chapters
- 00:05Title
- 01:20Preparation of Cells and Creating Wounds
- 02:50Monitor Cell Migration and Data Analysis
- 04:09Förster Resonance Enegry Transfer
- 07:17Data Analysis
- 08:34Results: MRP4 Regulation of Fibroblast Migration
- 09:40Conclusion
MRP4 regulates various cyclic nucleotide-dependent signaling events including a recently elucidated role in cell migration. We describe a direct, but multifaceted approach to unravel the downstream molecular targets of MRP4 resulting in identification of a unique MRP4 interactome that plays key roles in the fine-tuned regulation of fibroblast migration.
