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方法在成纤维细胞迁移的规律研究大分子复合物含MRP4，

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Developmental Biology

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JoVE Journal Developmental Biology

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

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方法在成纤维细胞迁移的规律研究大分子复合物含MRP4，

DOI:

10.3791/53973-v

•

10:43 min

•

May 19, 2016

•

Chandrima Sinha*^1,2, Kavisha Arora*¹, Anjaparavanda P. Naren²

¹Division of Pulmonary Medicine, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, ²Department of Physiology,University of Tennessee Health Science Center

Chapters

00:05Title
01:20Preparation of Cells and Creating Wounds
02:50Monitor Cell Migration and Data Analysis
04:09Förster Resonance Enegry Transfer
07:17Data Analysis
08:34Results: MRP4 Regulation of Fibroblast Migration
09:40Conclusion

Summary

Automatic Translation

MRP4调节各种循环依赖于核苷酸信号事件，包括细胞迁移最近阐明的作用。我们描述一个直接的，而是多方面的方法解开导致该起主要作用的成纤维细胞迁移的微调调控独特MRP4相互作用组鉴定MRP4下游分子的目标。

Tags

MRP4 Fibroblast Migration Cyclic AMP FRET Imaging High-content Microscopy Cell Migration Protein-protein Interactions F-actin

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