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Methods for the Study of Regeneration in Stentor
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Methods for the Study of Regeneration in Stentor

Methods for the Study of Regeneration in Stentor

DOI:
10.3791/57759-v

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08:48 min

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June 13, 2018

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, , ,
1Department of Biochemistry and Biophysics,University of California San Francisco

Chapters

  • 00:05Title
  • 00:37Stentor Culture Establishment
  • 04:24Regeneration Induction
  • 06:22Cell Regeneration Imaging and Analysis
  • 07:02Results: Representative Regeneration Analysis
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

The giant ciliate, Stentor coeruleus, is an excellent system to study regeneration and wound healing. We present procedures for establishing Stentor cell cultures from single cells or cell fragments, inducing regeneration by cutting cells, chemically inducing the regeneration of membranellar band and oral apparatus, imaging, and analysis of cell regeneration.

Tags

StentorRegenerationSingle-cell ModelCell BiologyChlamydomonasCell CultureCell TransferCell WashingCell FeedingClonal CultureCell CountingCell Passaging

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