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用于分析 念珠菌 Cdr1-mGFPHis分析的小尺度等离子膜制备
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Biologia
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JoVE Journal Biologia
Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
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用于分析 念珠菌 Cdr1-mGFPHis分析的小尺度等离子膜制备

DOI:
10.3791/62592-v

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09:44 min

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June 13, 2021

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, , , , ,
1Sir John Walsh Research Institute, Faculty of Dentistry,University of Otago, 2Department of Microbiology, Faculty of Medicine,Chulalongkorn University, 3School of Biological Sciences,University of Auckland

Capítulos

  • 00:04Introduction
  • 00:57Growing and Harvesting Yeast Cells
  • 02:26Isolation of Plasma Membranes
  • 04:14Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
  • 06:22Results: Analysis of Cdr1-mGFPHis Isolated from Plasma Membranes of Saccharomyces cerevisiae
  • 09:06Conclusion

Summary

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Tadução automática

本文提出了一个小规模的等离子膜隔离协议，用于描述在糖浆中过度表达的念珠菌ABC（ATP结合盒式）蛋白质Cdr1。 蛋白酶可切割的 C 端 mGFPHis 双标签，Cdr1 和标签之间有 16 残留链接器，旨在促进 Cdr1 的净化和洗涤剂筛选。

Tags

Plasma MembraneCandida AlbicansCdr1-mGFPHisMembrane ProteinSmall-scale IsolationATPaseDetergentCell HarvestingPre-cultureCell LysisCentrifugationHomogenization Buffer

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