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无需梯度测定器或分馏系统的多聚体分析
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JoVE Journal Bioquímica
Polysome Profiling without Gradient Makers or Fractionation Systems
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无需梯度测定器或分馏系统的多聚体分析

DOI:
10.3791/62680-v

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05:56 min

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June 01, 2021

•

,
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Capítulos

  • 00:04Introduction
  • 00:39Preparation of 7 to 47% Sucrose Gradients
  • 02:14Preparation of Yeast Cell Extracts
  • 03:21Centrifugation of Gradients
  • 03:49Fraction and Data Collection
  • 04:36Results: Polysome Profiling Without Gradient Makers or Fractionation Systems
  • 05:19Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了如何在不使用自动梯度制作器或梯度分级分离系统的情况下生成多聚体谱。

Tags

Polysome ProfilingGradient-freeSucrose GradientRibosome ActivityYeast Cell LysisRNA ExtractionRNA Quantification

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