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Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection
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Biologia
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JoVE Journal Biologia
Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection

Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection

DOI:
10.3791/62831-v

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06:13 min

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August 27, 2021

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, , , ,
1State Key Laboratory of Plant Genomics, Institute of Microbiology,Chinese Academy of Sciences, 2National Plant Gene Research Center, 3University of the Chinese Academy of Sciences

Capítulos

  • 00:05Introduction
  • 01:02Preparation of Feeding Chamber and Artificial Diet
  • 02:00Collection of Small Brown Planthopper (SBPH) Saliva
  • 02:56Concentration of the Collected Saliva
  • 03:58Results: Artificial Feeding and Saliva Collection from Laodelphax striatellus
  • 05:21Conclusion

Summary

Tadução automática

Tadução automática

The present protocol describes a method to collect sufficient saliva from piercing-sucking insects using an artificial medium. This is a convenient method for collecting insect saliva and studying salivary function on insect feeding behavior and vector-borne virus transmission.

Tags

Membrane Sandwich MethodLaodelphax StriatellusSaliva CollectionArtificial FeedingSaprophytic InsectsSucrose SolutionPlant Hopper LarvaeFeeding ChamberParaffin MembraneArtificial DietAluminum FoilSterile TubesCentrifugal Filter

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