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Tilpasning af gastrointestinale organoider til patogeninfektion og enkeltcellesekvens under biosikkerhedsniveau 3 (BSL-3) Betingelser
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
DOI:

07:59 min

September 10, 2021

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Capítulos

  • 00:04Introduction
  • 01:04Preparation of Single-Cell Solution and Preparation of Gel Beads-in-Emulsion Under Biosafety Level 3 Conditions
  • 05:47Results: Characterization of Host/Pathogen Interactions at a Single-Cell Level Using scRNAseq Technology
  • 07:11Conclusion

Summary

Tadução automática

Denne protokol beskriver, hvordan man inficerer menneskelige intestinale organoider fra enten deres apikale eller basolaterale side for at karakterisere værts /patogeninteraktioner på enkeltcelleniveau ved hjælp af encellet RNA-sekventering (scRNAseq) teknologi.

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