Journal
/
Neurociência
/
Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

DOI:
10.3791/62980-v

•

07:23 min

•

August 06, 2021

•

, , , ,
1Department of Pathology & Cell Biology; Vagelos College of Physicians and Surgeons,Columbia University, 2Department of Molecular Pharmacology and Therapeutics; Vagelos College of Physicians and Surgeons,Columbia University, 3Department of Neurology; Vagelos College of Physicians and Surgeons,Columbia University, 4The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain; Vagelos College of Physicians and Surgeons,Columbia University

Capítulos

  • 00:05Introduction
  • 00:45Laser Setup
  • 02:05Mouse Tail Vein Injection of Rose Bengal
  • 02:54Occlusion of Major Veins
  • 04:07Establishing the Number of Veins Occluded at Day 0 and Aftercare
  • 04:40Results: Analysis of Types and Timelines of Photo-Occlusion, Baseline and Experimental Laser Output Measurement, Ophthalmic Pathologies, and Quantification of OCT Images
  • 06:37Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe an optimized protocol for retinal vein occlusion using rose bengal and a laser-guided retinal imaging microscope system with recommendations to maximize its reproducibility in genetically modified strains.

Tags

Retinal Vein OcclusionMouse ModelVascular InjuryEdemaNon-invasiveTail VeiningRetinal Imaging MicroscopeLaser Control BoxRose BengalAnesthesiaPhenylephrineTropicamideProparacaine HydrochlorideCarprofen

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/62980?article_type=v

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat
check_url/pt/62980?article_type=v

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
check_url/pt/62980?article_type=v

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
check_url/pt/62980?article_type=v

Detection of Neuritic Plaques in Alzheimer’s Disease Mouse Model
check_url/pt/62980?article_type=v

Morphometric Analyses of Retinal Sections
check_url/pt/62980?article_type=v

Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
check_url/pt/62980?article_type=v

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
check_url/pt/62980?article_type=v

Establishment of a Rat Model of Superior Sagittal-Sinus Occlusion via a Thread-Embolism Method
check_url/pt/62980?article_type=v

Innovative Techniques and Future Directions in Stroke Research (Video) | JoVE
check_url/pt/62980?article_type=v

A Neonatal Rodent Model of Retroorbital Vein Injection

Read Article