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Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
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Medicina
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JoVE Journal Medicina
Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

DOI:
10.3791/63036-v

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05:57 min

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December 30, 2021

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, , , , , , ,
1Genetics Division,Universidade Federal de São Paulo (UNIFESP), 2TechLife Biotech,São Lucas Cell Therapy Group, 3Division of Plastic Surgery,Universidade Federal de São Paulo (UNIFESP)

Capítulos

  • 00:04Introduction
  • 01:21Processing of Lipoaspirate
  • 02:29Counting of the SVF Cells
  • 03:19Results: Morphology and Viability of ADSCs, bthe Subpopulation of Cells Positive for Stem Cell-Associated Markers in SVF, and the Differentiation Assay
  • 05:05Conclusion

Summary

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The present protocol describes an improved methodology for ADSC isolation resulting in a tremendous cellular yield with time gain compared to the literature. This study also provides a straightforward method for obtaining a relatively large number of viable cells after long-term cryopreservation.

Tags

Mesenchymal Stem CellsStromal Vascular FractionCryopreservationCell IsolationCell CharacterizationCell ViabilityCell CountingCell SuspensionCryoprotective MediumFreezing ProtocolLong-term Storage

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