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天然的に無秩序なタンパク質の自己会合の検出と特性評価のための常磁性緩和増強
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
DOI:

07:24 min

September 23, 2021

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Capítulos

  • 00:05Introduction
  • 00:49Conjugating the 3-Maleimido-PROXYL Nitroxide Spin Label
  • 01:38Preparing the NMR Sample for Measuring Intramolecular PRE
  • 02:11Setting Up the NMR Spectrometer and Experiment Specific Parameters
  • 03:51Setting Up the 1HNT2 Experiment
  • 04:58Making a Diamagnetic Sample by Reducing Spin-Label with Ascorbic Acid
  • 05:52Results: 1HNT2 Rates for Residues 171 – 264 of the Intrinsically Disordered Domain of EWSR1
  • 06:30Conclusion

Summary

Tadução automática

天然変性タンパク質の弱く一過性の分子間および分子内相互作用を検出するための常磁性緩和増強NMR分光法の適用のためのプロトコルが提示されています。

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