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提高可重复性以满足细胞外囊泡研究的最少信息 2018纳米颗粒跟踪分析指南
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
DOI:

08:52 min

November 17, 2021

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Capítulos

  • 00:04Introduction
  • 00:44Cleaning and Assembly of the Laser Module
  • 01:29Flushing Procedure for the Laser Module Prior to and Between Samples
  • 02:28Focusing and Positioning of the Laser Module
  • 03:34Loading Standards/Samples/Diluent into the Laser Module for Nanoparticle Tracking Analysis (NTA)
  • 04:28Sample Nanoparticle Tracking Analysis (NTA)
  • 06:06Re-Analysis of the Current Sample at Different Detection Threshold
  • 06:40Results: Effects of Camera Level and Detection Threshold on Measured Particle Size and Concentration of Filtered and Unfiltered Samples
  • 08:02Conclusion

Summary

Tadução automática

纳米颗粒跟踪分析(NTA)是一种广泛使用的表征细胞外囊泡的方法。本文重点介绍了NTA实验参数和对照,以及样品和稀释剂的统一分析和表征方法,这是补充MISEV2018和EV-TRACK提出的实验室之间可重复性指南所必需的。

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