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Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
 

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Article DOI: 10.3791/63157-v • 05:50 min • November 1st, 2021
November 1st, 2021 •
*1, *1, 1, 1, 1, 2,3, 1
1The Azrieli Faculty of Medicine, Bar-Ilan University, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Neuroscience, Yale University
* These authors contributed equally

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This protocol aims to measure the dynamic parameters (protrusions, retractions, ruffles) of protrusions at the edge of spreading cells.

Tags

Cell-edge Protrusion Dynamics Spreading Live-cell Microscopy Cell Migration Critical Proteins Signaling Mechanisms Cell Motility Hydrochloric Acid Solution Glass Bottom Dish Fibronectin Incubation BSA Solution PBS Solution Denaturation Experimental Setup
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