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使用活细胞显微镜测量扩散过程中的细胞边缘突起动力学

JoVE Journal
Biologia

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JoVE Journal Biologia

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

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使用活细胞显微镜测量扩散过程中的细胞边缘突起动力学

DOI:

10.3791/63157-v

•

05:50 min

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November 01, 2021

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Nikola Lukic*¹, Trishna Saha*¹, Stefanie Lapetina, Michal Gendler, Gilad Lehmann, Anthony J. Koleske³, Hava Gil-Henn

¹The Azrieli Faculty of Medicine,Bar-Ilan University, ²Department of Molecular Biophysics and Biochemistry,Yale University, ³Department of Neuroscience,Yale University

Capítulos

00:04Introduction
00:47Glass-Bottom Dish Coating
01:48Preparation of Cells for Imaging
02:27Microscope Setup and Imaging
03:23Image Analysis
04:29Results: Cell-Edge Protrusion Assay at the Edge of Spreading Cells
05:08Conclusion

Summary

Tadução automática

该协议旨在测量扩散细胞边缘突起的动态参数（突起，缩回，褶皱）。

Tags

Keywords: Cell-edge Protrusion Cell Migration Live-cell Microscopy Cell Motility Fibronectin BSA Trypsin Hemacytometer Phase Contrast Microscopy

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