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培养的果蝇脑外植体中的神经干细胞再激活

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

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培养的果蝇脑外植体中的神经干细胞再激活

DOI:

10.3791/63189-v

•

05:54 min

•

May 18, 2022

•

Cami Naomi Keliinui, Susan E. Doyle, Sarah E. Siegrist

¹Biology department,University of Virginia

Capítulos

00:04Introduction
00:35Drosophila Larvae Collection
01:23Culture Media and Tool Preparation
02:03Dissections and Brain Cultures
02:57Proliferation Assay, Brain Fixation, and Antibody Staining
04:24Results: Confocal Imaging of Stained Brain Cells
05:19Conclusion

Summary

Tadução automática

已经建立了一种在培养的果蝇脑外植体中重新激活静止神经干细胞的方法。使用这种方法，全身信号的作用可以从组织内在信号中分离出来，调节神经干细胞的静止，进入和退出。

Tags

Neural Stem Cells Drosophila Brain Explants Stem Cell Reactivation Extrinsic Cues Cell Cycle Dissection Schneider’s Culture Medium 5-ethynyl-2′-deoxyuridine (EdU)

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