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JoVE Journal Biochemistry
In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
 

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Article DOI: 10.3791/63210-v • 08:48 min • November 5th, 2021
November 5th, 2021 •
1,2, 1, 1, 3, 1,2, 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

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We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.

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Keywords: Trypanosoma Cruzi In Vitro Drug Screening High-throughput Colorimetric Assay Parasite Life Cycle Stages Epimastigotes Trypomastigotes Vero Cells Beta-galactosidase Chagas Disease Drug Discovery
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