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使用光敏转移素对活体植入前小鼠胚胎中的微管细胞骨架进行时空亚细胞操作
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins
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使用光敏转移素对活体植入前小鼠胚胎中的微管细胞骨架进行时空亚细胞操作

DOI:
10.3791/63290-v

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08:13 min

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November 30, 2021

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, , , ,
1Australian Regenerative Medicine Institute,Monash University, 2School of BioSciences,The University of Melbourne

Capítulos

  • 00:04Introduction
  • 00:53Drug and Imaging Dish Preparation
  • 02:21Live Imaging and PST-1P Photoactivation
  • 05:20Results: Effect of PST-1P Treatment on the Embryos
  • 07:31Conclusion

Summary

Tadução automática

Tadução automática

典型的微管抑制剂广泛用于基础和应用研究，对细胞具有深远的影响。最近，光敏素作为一类光开关微管抑制剂出现，能够对微管进行瞬时，可逆，时空精确操作。该分步实验方案详细介绍了迁光素在3D活体植入前小鼠胚胎中的应用。

Tags

Keywords: Spatiotemporal Subcellular ManipulationMicrotubule CytoskeletonPreimplantation Mouse EmbryoPhotostatinsLight-switchable Drugs3D Physiological SystemsMicrotubule GrowthStock SolutionWorking SolutionKSOMLive ImagingEnvironmental ChamberEB3-dTomato CometsZ-stackLaser PowerDigital OffsetBackground NoisePinholePixel ResolutionPixel Dwell Time

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