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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

DOI:
10.3791/63300-v

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06:08 min

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October 17, 2022

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, , ,
1Department of Digital Anti-Aging Health Care,Inje University, 2Micro device Lab Co., Ltd, 3Biohealth Products Research Center (BPRC),Inje University, 4Department of Biomedical Engineering,Inje University

Capítulos

  • 00:04Introduction
  • 00:45Microuidic Acoustophoresis Chip Design and Fabrication
  • 01:23Bacterial Strains and Culture
  • 01:56Microbeads and Immobilization of Aptamer onto Microbeads
  • 03:10Acoustophoresis Setup and Operation
  • 04:19Results: Using Microfluidics to Capture GN Bacteria from Culture Samples at High Speed
  • 05:26Conclusion

Summary

Tadução automática

Tadução automática

This paper describesthe fabrication and operation of microfluidic acoustophoretic chips using the microfluidic acoustophoresis technique and aptamer-modified microbeads that can be used for fast, efficient isolation of Gram-negative bacteria from a medium.

Tags

Microfluidic AcoustophoresisGram-negative Bacteria SeparationAptamer Affinity BeadsFlowthrough SeparationCell IsolationMicrofluidic DevicePZTBiotinylated DNA AptamerStreptavidin-coated MicrobeadsLuria-Bertani MediumCentrifugationTris-HCl BufferBSA

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