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Etikettfri icke-linjär optik för studier av tubulinberoende defekter i centrala Myelin
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
DOI:

08:07 min

March 24, 2023

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Chapters

  • 00:04Introduction
  • 01:02Microscope Settings
  • 02:00Microscope Preliminary Controls
  • 03:00Vibratome Sectioning
  • 04:00Transfer to the Microscope and Imaging
  • 05:15Processing of the Cerebellum
  • 05:35Results: Visualization of Tubulin-Dependent Defects in Central Myelin
  • 06:38Conclusion

Summary

Automatic Translation

I den här artikeln presenterar vi ett protokoll för att detektera mikrotubulibelastade oligodendrocyter i en modell av tubulinopati genom en enkel, innovativ andra harmonisk generationens mikroskopimetod.

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