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Laser Nanosurgery of Cerebellar Axons In Vivo
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JoVE Journal Neuroscience
Laser Nanosurgery of Cerebellar Axons In Vivo

Laser Nanosurgery of Cerebellar Axons In Vivo

DOI:
10.3791/51371-v

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09:25 min

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July 28, 2014

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, ,
1European Laboratory for Non-Linear Spectroscopy,University of Florence, 2National Institute of Optics,National Research Council, 3Department of Physics and Astronomy,University of Florence, 4International Center for Computational Neurophotonics (ICON Foundation)

Chapters

  • 00:05Title
  • 02:02Axonal Labeling
  • 04:42Optical Window on the Cerebellar Cortex
  • 06:29In Vivo Multi-photon Laser Axotomy
  • 07:56Results: Laser Nanosurgery of Cerebllar Axons In Vivo
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

Two-photon imaging, coupled to laser nanodissection, are useful tools to study degenerative and regenerative processes in the central nervous system with subcellular resolution. This protocol shows how to label, image, and dissect single climbing fibers in the cerebellar cortex in vivo.

Tags

Laser NanosurgeryCerebellar AxonsIn VivoTwo-photon Fluorescence ImagingNeuronal RegenerationClimbing FibersOlivocerebellar NeuronsAnterograde TracingDextran-conjugated DyeCranial WindowTi:Sapphire Laser

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