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JoVE Journal Neuroscience
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
 

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

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Summary
Automatic Translation

This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.

Tags

Keywords: Cerebellar Granule Neurons Neuronal Death Neurodegeneration Primary Cell Culture Excitotoxicity Oxidative Stress DNA Damage Developmental Events Neuronal Activity Morphogenesis Growth Factors Mouse Brain Dissection Cerebellum Isolation Meningeal Removal
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