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小脑轴突的激光Nanosurgery<em>在体内</em
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Neuroscience
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JoVE Journal Neuroscience
Laser Nanosurgery of Cerebellar Axons In Vivo
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小脑轴突的激光Nanosurgery<em>在体内</em

DOI:
10.3791/51371-v

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09:25 min

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July 28, 2014

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, ,
1European Laboratory for Non-Linear Spectroscopy,University of Florence, 2National Institute of Optics,National Research Council, 3Department of Physics and Astronomy,University of Florence, 4International Center for Computational Neurophotonics (ICON Foundation)

Chapters

  • 00:05Title
  • 02:02Axonal Labeling
  • 04:42Optical Window on the Cerebellar Cortex
  • 06:29In Vivo Multi-photon Laser Axotomy
  • 07:56Results: Laser Nanosurgery of Cerebllar Axons In Vivo
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

双光子成像，其耦合到激光nanodissection，是有用的工具来研究退化和再生过程中对中枢神经系统具有亚细胞分辨率。该协议显示了如何标记，图像，并剖析单个登山纤维在体内小脑皮质。

Tags

Keywords: Laser NanosurgeryCerebellar AxonsIn VivoTwo-photon Fluorescence ImagingNeuronal RegenerationClimbing FibersOlivocerebellar NeuronsAnterograde TracingDextran-conjugated DyeCranial WindowTi:Sapphire Laser

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