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Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
JoVE Journal
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JoVE Journal Pesquisa do Câncer
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
DOI:

08:00 min

March 30, 2022

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Capítulos

  • 00:05Introduction
  • 00:49Cell Culture
  • 01:17Cell Seeding and Assembly
  • 04:09Impedance Measurement
  • 04:44Detachment and Cell Collection
  • 05:54Results: Detection and Capture of Invasive Cell Subpopulations in Real-Time
  • 07:34Conclusion

Summary

Tadução automática

We describe an approach to detect and capture invasive cell subpopulations in real-time. The experimental design uses Real-Time Cellular Analysis by monitoring changes in the electric impedance of cells. Invasive cancer, immune, endothelial or stromal cells in complex tissues can be captured, and the impact of co-cultures can be assessed.

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