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Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots
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Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

DOI:
10.3791/63533-v

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05:51 min

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March 31, 2022

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, , , ,
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República (UdelaR), 2Laboratorio de Señalización Celular y Nanobiología,Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

Capítulos

  • 00:04Introduction
  • 00:41Preparation of the Sample for Nanoindentation Experiments
  • 01:14Indentation Protocol
  • 03:16Results: Analysis of Force-Displacement Curves and Histograms
  • 05:13Conclusion

Summary

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The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit).

Tags

Atomic Force MicroscopyEpidermal CellsArabidopsis RootsPhysical PropertiesCell WallIn VivoNon-invasiveSubcellular ResolutionSilicone GluePBSCantileverDeflection SensitivitySpring ConstantThermal TuneForce Curves

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