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細胞および組織におけるグルコース取り込みの間接的尺度としての細胞外グルコース枯渇 Ex vivo
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
DOI:

10:35 min

April 06, 2022

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Capítulos

  • 00:04Introduction
  • 01:043T3‐L1 Cell Culture and Maintenance
  • 01:49Starvation of 3T3‐L1 Cells
  • 02:22Preparation of FD‐Glucose Solutions with Different Concentrations
  • 04:19Treating Starved 3T3‐L1 Cells
  • 05:03Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells
  • 06:00Protein‐Based Normalization of Intercellular Glucose Uptake
  • 06:35Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs
  • 07:59Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion
  • 09:39Conclusion

Summary

Tadução automática

蛍光標識されたグルコースの細胞外枯渇は、グルコース取り込みと相関し、摘出した臓器および細胞培養物におけるグルコース取り込みのハイスループットスクリーニングに使用することができる。

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