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Indre mitokondriemembranfølsomhed over for Na+ afslører delvist segmenterede funktionelle CoQ-puljer
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
DOI:

05:27 min

July 20, 2022

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Chapters

  • 00:04Introduction
  • 00:40Measuring the Activity of Complex I + Complex III
  • 02:39Measuring the Activity of Complex II + Complex III
  • 04:04Results: Cytochrome c Reduction by Mouse Liver Mitochondrial Membranes
  • 04:54Conclusion

Summary

Automatic Translation

Denne protokol beskriver et komparativt assay ved hjælp af mitokondrielle komplekse aktiviteter CI + CIII og CII + CIII i nærvær eller fravær af Na + for at studere eksistensen af delvist segmenterede funktionelle CoQ-puljer.

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