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Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
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Biochemistry
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JoVE Journal Biochemistry
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

DOI:
10.3791/63729-v

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05:27 min

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July 20, 2022

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1Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, 2Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

Chapters

  • 00:04Introduction
  • 00:40Measuring the Activity of Complex I + Complex III
  • 02:39Measuring the Activity of Complex II + Complex III
  • 04:04Results: Cytochrome c Reduction by Mouse Liver Mitochondrial Membranes
  • 04:54Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a comparative assay, using mitochondrial complex activities CI+CIII and CII+CIII in the presence or absence of Na+, to study the existence of partially segmented functional CoQ pools.

Tags

Na+ SensitivityMitochondrial MembraneCoQ PoolsElectron TransferLipid DropsCytochrome CMalonateRotenoneNADHAntimycin ASuccinate

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