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Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections
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Neurociência
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JoVE Journal Neurociência
Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections

Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections

DOI:
10.3791/63804-v

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10:53 min

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April 20, 2022

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,
1Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Cell Biology and Physiology,University of North Carolina, Chapel Hill

Capítulos

  • 00:05Introduction
  • 00:55Immunostaining
  • 04:42Confocal Imaging
  • 05:57Image Analysis
  • 09:22Results: Analysis of Astrocyte Territory Volume and Astrocyte Territory Overlap or Tiling
  • 10:27Conclusion

Summary

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This protocol describes methods for sectioning, staining, and imaging free-floating tissue sections of the mouse brain, followed by a detailed description of the analysis of astrocyte territory volume and astrocyte territory overlap or tiling.

Tags

AstrocyteAstrocyte TerritoryAstrocyte MorphologyImmunohistochemistryAstrocyte DevelopmentAstrocyte-astrocyte InteractionTriton XBlocking SolutionPrimary AntibodySecondary Antibody

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