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JoVE Journal Neuroscience
Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis
 

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Article DOI: 10.3791/63829-v • 08:20 min • June 2nd, 2022
June 2nd, 2022 •
1, 2, 1, 1,2
1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic, Universitat de Barcelona

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Autoimmune encephalitis is a new category of antibody-mediated diseases of the central nervous system. Hippocampal neurons can be used to discover and characterize these antibodies. This article provides a protocol for primary cell culture and immunostaining to determine autoantibodies in the serum and cerebrospinal fluid of patients.

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Keywords: Hippocampal Neuronal Cultures Autoimmune Encephalitis Pathogenic Antibodies Surface Antigens Intracellular Antigens Embryonic Brain Dissection Hippocampus Isolation Enzymatic Dissociation Cell Culture
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