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Imunologia e Infecção

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经口喂养和显微注射对成年蚊子进行实验性病毒感染

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

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经口喂养和显微注射对成年蚊子进行实验性病毒感染

DOI:

10.3791/63830-v

•

08:02 min

•

July 28, 2022

•

Fei Wang*¹, Cihan Yang*^1,2, Shunlong Wang², Qun Wu², Christabel Ochieng², Zhiming Yuan², Han Xia²

¹Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology,Chinese Academy of Sciences, ²University of Chinese Academy of Sciences

Capítulos

00:04Introduction
00:58Performing Oral Infection
02:32Intrathoracic Inoculation
04:02Viral Injection Under a Dissecting Microscope
04:53Processing of the Infected Female Mosquitoes
06:42Results: Determination of Viral Infection in Salivary Glands of Aedes aegypti
07:35Conclusion

Summary

Tadução automática

该方法包括经口喂养和胸内注射感染，可以有效评估中肠和/或唾液腺屏障对虫媒病毒感染的影响。

Tags

Keywords: Viral Infection Mosquitoes Oral Feeding Microinjection Aedes Aegypti Arbovirus Vector Competence Dengue Virus Zika Virus Artificial Feeding System Anesthesia Incubation Intrathoracic Inoculation Virus Dilution Microinjection Needles

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