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Bioengineering

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JoVE Journal Bioengineering
Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
 

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Article DOI: 10.3791/63862-v • 06:19 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 1,2, 1,2
1HKUST-Shenzhen Research Institute, 2Department of Chemistry, The Hong Kong University of Science and Technology

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The present protocol describes a convenient approach to integrating optical trapping and surface-enhanced Raman spectroscopy (SERS) to manipulate plasmonic nanoparticles for sensitive molecular detection. Without aggregating agents, the trapping laser assembles plasmonic nanoparticles to enhance the SERS signals of target analytes for in situ spectroscopic measurements.

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Keywords: Optical Trapping Plasmonic Nanoparticles Surface-enhanced Raman Spectroscopy SERS-active Nanoparticle Assembly Analyte Detection Biomarker Detection Microfluidic System 532-nanometer Laser Liquid-nitrogen-cooled Charge-coupled Device Camera Silver Nanoparticles DSNB Solution Raman Excitation Source Laser
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