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用于心胸外科的青春期前小鼠幼崽的麻醉和插管

JoVE Journal
Biologia

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JoVE Journal Biologia

Anesthesia and Intubation of Preadolescent Mouse Pups for Cardiothoracic Surgery

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用于心胸外科的青春期前小鼠幼崽的麻醉和插管

DOI:

10.3791/64004-v

•

09:47 min

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June 02, 2022

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Jianxin Wu*¹, Amy M. Nicks², Justin J. Skowno⁴, Michael P. Feneley², Robert M. Graham², Siiri E. Iismaa*^1,2

¹Molecular Cardiology and Biophysics Division,Victor Chang Cardiac Research Institute, ²St Vincent’s Clinical School,University of New South Wales Sydney, ³Department of Anaesthesia,The Children’s Hospital at Westmead, ⁴School of Child and Adolescent Health,University of Sydney

Capítulos

00:05Introduction
01:00Preparation of Instruments and Intubation of 10-Day-Old Mice
03:09Myocardial Infarction Surgery of 10-Day-Old Mice
05:13Post-Surgery Assessment of Infarct Size
07:04Results: Anesthesia Regimen, Number of Intubation Attempts, and Post-Procedure Survival of 10-Day-Old Mouse Pups
08:58Conclusion

Summary

Tadução automática

>7 日龄小鼠的心胸外科模型需要插管，但这对青春期前（8-14 天大）小鼠幼崽具有挑战性，并且关于插管麻醉方案的信息很少。在这里，我们提出了氯胺酮/甲苯噻嗪/阿托品在 10 天大的 C57BL/6J 小鼠幼崽中的剂量方案，允许气管插管，同时最大限度地减少动物死亡率。

Tags

Anesthesia Intubation Preadolescent Mouse Pups Cardiothoracic Surgery Endotracheal C57 Black Six J 10-day Old Cannula Laryngoscope Tracheal Ventilator Spontaneous Breathing

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