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Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
 

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Article DOI: 10.3791/64069-v • 08:50 min • June 9th, 2022
June 9th, 2022 •
1,2, 1,3, 1,2,4
1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto

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We describe the surgical technique and decellularization process for composite rat hindlimbs. Decellularization is conducted using low-concentration sodium dodecyl sulfate through an ex vivo machine perfusion system.

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Keywords: Procurement Decellularization Rat Hindlimbs Ex Vivo Perfusion-based Bioreactor Vascularized Composite Allotransplantation Immunosuppression Tissue Immunogenicity Femoral Vessels Cannulation Heparinized Saline Bioreactor Peristaltic Tubing
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