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JoVE Journal Neuroscience
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
 

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022
August 1st, 2022 •
*1,2, *1,2, 1,2, 1,2, 3, 3,7, 7, 4,5,6, 4,5,6, 3, 4,5,6, 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally

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Resumo
Automatic Translation

This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.

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Keywords: Whole-brain Single-cell Imaging Analysis Intact Neonatal Mouse Brain MRI Tissue Clearing Light-sheet Microscopy Image Analysis Nuclei Quantification Computational Pipeline Preprocessing Cell Detection Imaging Time Computational Resources Sample Mounting Sample Orientation Zoom Magnification Light Sheet Numerical Aperture Dynamic Focusing Laser Power Light Sheet Width Tile Overlap Conda NuMorph MATLAB Image Processing Intensity Adjustment
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