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Erstellung eines Kniegelenk-on-a-Chips zur Modellierung von Gelenkerkrankungen und zur Erprobung von Medikamenten
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1Department of Orthopaedic Surgery,University of Pittsburgh School of Medicine, 2Department of Bioengineering,University of Pittsburgh Swanson School of Engineering, 3Department of Neurobiology,University of Pittsburgh School of Medicine, 4Department of Orthopaedic Surgery,Stanford University, 5Department of Microbiology, Immunology, and Genetics,University of North Texas Health Science Center, 6Department of Bioengineering,Stanford University, 7McGowan Institute for Regenerative Medicine,University of Pittsburgh School of Medicine, 8The Chinese University of Hong Kong
Capítulos
- 00:05Introduction
- 00:43Engineering the Tissue Components
- 05:49Establishing the Minijoint Chip
- 08:13Individual Tissue Collection
- 09:35Results: Establishment of a Miniature Synovial Joint-Mimicking Microphysiological System
- 12:07Conclusion
Wir stellen detaillierte Methoden zur Generierung von vier Gewebetypen aus humanen mesenchymalen Stammzellen vor, die zur Rekapitulation von Knorpel, Knochen, Fettpolster und Synovia im menschlichen Kniegelenk verwendet werden. Diese vier Gewebe werden in einen maßgeschneiderten Bioreaktor integriert und durch Mikrofluidik miteinander verbunden, so dass ein Kniegelenk-on-a-Chip entsteht.
