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A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
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Biologia
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JoVE Journal Biologia
A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

DOI:
10.3791/64201-v

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03:55 min

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June 27, 2022

•

,
1Department of Microbiology, School of Life Science and Technology,China Pharmaceutical University, 2National Experimental Teaching Demonstration Center of Biopharmaceuticals,China Pharmaceutical University

Capítulos

  • 00:04Introduction
  • 01:02Bacterial Strains
  • 01:48H2S Detection Assay
  • 02:50Results: Visual Assessment of H2S Production
  • 03:29Conclusion

Summary

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Here, we present a protocol to detect hydrogen sulfide producing bacteria with a modified protocol used for bismuth sulfide (BS) precipitation. The key advantages of this method are that it is easy to evaluate and does not require specialized equipment.

Tags

Hydrogen SulfideBacteriaBismuth SulfidePrecipitation96-well Microtiter PlateFusobacterium NucleatumProteus VulgarisSalmonella Paratyphi BEnterococcus FaecalisPseudomonas AeruginosaSalmonella Paratyphi AStaphylococcus AureusAeromonas HydrophilaKlebsiella Pneumoniae

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