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JoVE Journal Neuroscience
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
 

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

Article DOI: 10.3791/64224-v • 06:45 min • August 4th, 2022
August 4th, 2022 •
1, 2,3, 4, 2,3, 1
1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

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The present protocol describes making a large (6 x 3 mm2) cranial window using food wrap, transparent silicone, and cover glass. This cranial window allows in vivo wide-field and two-photon calcium imaging experiments in the same mouse.

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In Vivo Wide-field Two-photon Calcium Imaging Mouse Large Cranial Window Neural Activities Glial Activities Behavior Macroscopic Level Microscopic Level Small Cranial Window Dental Drill Scalpel Blunt Tip Bone Scraping Dura Mater Removal Glass Pipette U-shaped Needle Stereo Microscope Bleeding ACSF (artificial Cerebrospinal Fluid) Gelatin Sponge PVDC Wrap
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