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应用于 果蝇 微生物组的 96 孔板形式的快速菌落形成单位计数
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome
DOI:

12:55 min

January 13, 2023

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Capítulos

  • 00:04Introduction
  • 01:28Homogenizing Flies Individually in a 96-Well PCR Plate
  • 04:13Serial Dilution of Fly Homogenate and Spotting onto CFU Plates
  • 06:03Quantification of CFUs
  • 06:44Automated Colony Counting Using the Count-On-It Suite
  • 09:57Results: Enumeration of CFUs Using Automated Colony Counting
  • 12:10Conclusion

Summary

Tadução automática

该方法使用96孔板形式量化微生物丰度,以板菌落形成单位(CFU),并应用于整个果蝇匀浆样品中的 果蝇 微生物组。CFU使用此处提供的自动图像分析软件进行计数。

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