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离体 尿路上皮细胞中机械活化的CA2+ 瞬变分析
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JoVE Journal Medicina
Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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离体 尿路上皮细胞中机械活化的CA2+ 瞬变分析

DOI:
10.3791/64532-v

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05:35 min

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September 28, 2022

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, ,
1Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh, 2Department of Cell Biology,University of Pittsburgh

Capítulos

  • 00:04Introduction
  • 00:31Fabrication of Micropipettes
  • 00:56In Situ Transduction and Isolation of Bladder Mucosa
  • 01:41Mechanical Stimulation of Individual Urothelial Cells and Ca2+ Imaging
  • 03:16Data Analysis
  • 04:03Results: Analysis of Mechano-Activated Ca2+ Transients in Urothelial Cells
  • 04:49Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了一种使用荧光Ca2 + 传感器GCaMP5G评估天然尿路上皮细胞中机械激活离子通道功能的方法。

Tags

Ex VivoMechanically-activatedCa2+ TransientsUrothelial CellsMicropipettesBladderUrethraCalcium Ion TransientsElectrophysiology

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